Jovian

Jovian performs virus-focused metagenomic analysis of Illumina data to generate annotated scaffolds, quality metrics, and interactive reports for clinical diagnostics and surveillance.

Key Features:

• Scaffold generation and annotation: Produces scaffolds with taxonomic classification and pertinent annotations.
• Quality assessment metrics: Calculates coverage depth, average GC content, localization of open reading frames, and minority single nucleotide polymorphisms.
• Metadata integration: Incorporates host and disease metadata into analysis outputs.
• Interactive reporting: Generates interactive web-based reports with an audit trail.
• Cross-platform reproducibility: Produces identical results with matching MD5sums across grid-computers, high-performance compute servers, and Windows10 laptops at different institutes.

Scientific Applications:

• Clinical diagnostics: Identifies viral pathogens in clinical samples, as validated by comparison with commercial online reference tools using viral gastroenteritis samples.
• Public health surveillance: Enhances the breadth of surveillance and testing programs to foster more effective public health interventions.
• Privacy-compliant deployment: Addresses privacy legislation and laboratory certification protocol requirements for clinical metagenomics.

Methodology:

Jovian is a metagenomic analysis workflow for Illumina data that generates scaffolds with taxonomic classification and quality metrics; specific computational steps and algorithms are not explicitly detailed in the sources.