K-Pro

K-Pro compiles experimental kinetic and thermodynamic data for monomeric proteins that fold via a two-state mechanism to support quantitative studies of protein folding kinetics and misfolding-related phenotypes.

Key Features:

• Data Collection and Curation: Collects and curates kinetic data from experimental studies.
• Extensive Dataset: Houses 1,529 records corresponding to 62 proteins and 65 structures and includes kinetic parameters such as the logarithm of folding and unfolding rates, Tanford's β values, and ϕ values.
• Thermodynamic Parameters: Incorporates thermodynamic data associated with kinetic measurements when available.
• Integration with Key Databases: Cross-links entries with the Protein Data Bank (PDB), UniProt, and PubMed.

Scientific Applications:

• Protein folding kinetics: Provides curated kinetic parameters for quantitative analysis of folding and unfolding rates.
• Mechanistic studies: Enables investigation of thermodynamic and kinetic aspects of two-state folding mechanisms.
• Misfolding disorders: Supports studies on mechanisms underlying human disorders caused by protein misfolding.
• Genotype–phenotype analysis: Facilitates exploration of relationships between genetic variations and phenotypic outcomes using data on proteins and their mutants.

Methodology:

Collects and curates experimental kinetic data, annotates kinetic parameters (logarithm of folding and unfolding rates, Tanford's β, and ϕ values), incorporates available thermodynamic measurements, and cross-links records to PDB, UniProt, and PubMed.