Kablammo

Kablammo generates interactive, vector-based visualizations of BLAST (Basic Local Alignment Search Tool) sequence alignments to aid interpretation of structural variation, shared protein domains, and genome misassembly in genomic analyses.

Key Features:

• BLAST alignment input: Accepts BLAST-generated sequence alignment data as the primary input for visualization.
• Interactive visualizations: Produces dynamic visual representations that enable interactive exploration of alignment features.
• Vector-based graphics: Maps alignments to vector graphics to produce high-quality, scalable images suitable for analysis and publication.
• Biological feature highlighting: Visualizes shared protein domains, chromosome structural modifications, and genome misassembly within alignments.
• Alignment segment annotation: Provides detailed parameters and annotations for individual alignment segments.

Scientific Applications:

• Structural variation analysis: Facilitates identification and interpretation of structural variations within genomes from alignment patterns.
• Genome misassembly detection: Aids detection and visualization of potential genome misassemblies revealed by BLAST alignments.
• Functional genomics: Supports identification of shared protein domains to inform functional annotation and comparative analyses.
• Cytogenetics and comparative genomics: Illustrates chromosome structural modifications and comparative alignment patterns across species or strains.

Methodology:

Takes BLAST-generated alignment data and converts it into a format that supports interactive exploration by mapping sequence alignment coordinates onto vector graphics and annotating each alignment segment with detailed parameters.