KBWS

KBWS provides proxy client access to 42 major bioinformatics web services within the EMBASSY/EMBOSS framework to enable sequence alignment, phylogenetic analysis, protein cellular localization prediction, and RNA secondary-structure prediction.

Key Features:

• EMBASSY/EMBOSS integration: Implemented as an EMBASSY package to integrate with the EMBOSS framework and enable interoperability with other EMBOSS tools.
• Proxy client access to web services: Provides proxy client tools that access 42 major bioinformatics web services for remote analyses.
• Local and multiple sequence alignment: Includes functionality for local and multiple sequence alignment for comparative sequence analyses.
• Phylogenetic analysis: Supports phylogenetic analysis for construction of evolutionary trees from sequence data.
• Protein and RNA structure prediction: Provides prediction of protein cellular localization and RNA secondary structures.
• SOAP/WSDL support: Implements SOAP services in Perl exposed via WSDL files and supports RPC Encoded and Document/literal styles.

Scientific Applications:

• Sequence analysis: Enables alignment and comparative sequence analyses using local and multiple sequence alignment tools.
• Structural prediction: Supports prediction of protein cellular localization and RNA secondary structure for functional inference.
• Evolutionary and phylogenetic studies: Facilitates construction of phylogenetic trees and analysis of evolutionary relationships from sequence data.
• Workflow integration: Combines multiple web-based services into unified analysis pipelines for multi-step bioinformatics workflows.

Methodology:

Implemented in C as an EMBASSY package for EMBOSS; SOAP services are implemented in Perl and accessible via WSDL, supporting RPC Encoded and Document/literal styles.