Kinase SARfari BLAST Search

Kinase SARfari BLAST Search performs BLAST sequence alignments of query sequences against a curated kinase domain dataset to identify homologous kinases and link them to ChEMBL bioactivity, binding, functional and ADMET data.

Key Features:

• Integration with ChEMBL: Alignments are associated with ChEMBL data, which includes over 5.4 million bioactivity measurements for more than 1 million compounds and 5200 protein targets, encompassing binding, functional and ADMET annotations.
• Curated Data: Kinase SARfari kinase domain entries are manually abstracted from primary literature and subsequently curated and standardized.
• BLAST-based Alignment: Sequence comparisons use the Basic Local Alignment Search Tool (BLAST) algorithm to match user queries against the Kinase SARfari kinase domain database.
• Sequence-to-bioactivity mapping: Alignment hits are mapped to associated target and bioactivity information to relate sequence similarity to chemical biology data.

Scientific Applications:

• Drug Discovery: Identify potential kinase targets and relate sequence similarity to known compound bioactivities for target prioritization.
• Chemical Biology Research: Support functional inference and hypothesis generation by comparing kinase sequences to a curated set of domain annotations and bioactivity links.
• Protein Target Analysis: Explore relationships among kinase sequences and their annotated targets across over 5200 protein entries to study functional and evolutionary similarities.

Methodology:

Sequence alignments are performed using the Basic Local Alignment Search Tool (BLAST) against a Kinase SARfari-curated kinase domain database enriched with ChEMBL data.