KOnezumi-AID

KOnezumi-AID facilitates gRNA design for Target-AID-mediated genome editing to create multiplex knockouts by inducing premature termination codons or exon skipping.

Key Features:

• Multiplex knockout design: Generates experiment-ready gRNA designs for simultaneous knockout of multiple genes using base editing without DNA double-strand breaks.
• Gene knockout strategies: Supports knockout by inducing premature termination codons or promoting exon skipping.
• Batch processing: Enables rapid and precise gRNA design for large-scale genome editing projects such as CRISPR screening.
• Species support: Generates gRNA designs for both mouse and human genomes.

Scientific Applications:

• CRISPR screening: Supports large-scale genome editing projects requiring rapid and precise gRNA design for multiple genes.
• Multiplex gene knockout: Enables simultaneous knockout of multiple genes while avoiding chromosomal rearrangements associated with DNA double-strand breaks.

Methodology:

KOnezumi-AID is a command-line tool that automates gRNA design for Target-AID-mediated base editing.