KymoRod

KymoRod performs automated kinematic analysis of rod-shaped plant organs, quantifying curvature, growth anisotropy, and relative elemental growth rates to support multiscale studies of organ growth.

Key Features:

• Automated kinematic analysis: Provides automated quantification of growth dynamics in rod-shaped organs such as roots and hypocotyls.
• Spatio-temporal data acquisition: Captures spatio-temporal profiles of curvature, growth anisotropy, and relative elemental growth rates over time.
• Handling complex movements: Analyzes organs undergoing significant morphological changes and nontrivial movement patterns.
• Versatile experimental conditions: Documents growth patterns across conditions including normal growth, exposure to growth-inhibiting drugs, and mechano-sensing mutants of Arabidopsis thaliana.

Scientific Applications:

• Multiscale modeling: Provides quantitative inputs for developing and validating multiscale models of organ growth.
• Growth-dynamics analysis: Enables detailed study of curvature evolution, anisotropic growth, and elemental growth-rate distributions in rod-shaped organs.
• Phenotyping under perturbations: Supports comparison of growth responses to chemical treatments and genetic perturbations, including mechano-sensing mutants of Arabidopsis thaliana.

Methodology:

Implemented in MATLAB, KymoRod analyzes the evolution of growth patterns in rod-shaped organs without requiring surface markers.