LipidQuant 1.0

LipidQuant 1.0 automates data processing for lipidomic quantitation workflows that integrate lipid class separation with hydrophilic interaction liquid chromatography (HILIC) or supercritical fluid chromatography (SFC) and high-resolution mass spectrometry for accurate lipid identification and quantitation.

Key Features:

• Automated Workflow: Automates processing from raw data to final reporting.
• Lipid Class Separation: Optimized for HILIC and SFC to enable co-ionization of lipid class internal standards with their respective analytes.
• High-Resolution Mass Spectrometry Integration: Integrates with high-resolution MS to provide detailed molecular information for lipid identification and quantitation.
• Comprehensive Data Processing Steps: Performs lipid identification using advanced algorithms, quantitation leveraging class-specific internal standards, isotopic correction to address interferences, and generation of comprehensive result reports.

Scientific Applications:

• Human serum lipidomics: Supports detailed lipidomic analysis of human serum samples.
• Clinical and biomedical research: Applicable to studies investigating disease mechanisms and biomarker discovery.
• Demonstrated application: Applied in a small cohort study of human serum samples.

Methodology:

Separation of lipid classes prior to mass spectrometric detection using HILIC or SFC to co-ionize internal standards with analytes, lipid identification via advanced algorithms, quantitation using internal standards, isotopic correction, and generation of result reports.