LipidSuite

LipidSuite performs preprocessing, statistical differential analysis, and enrichment analysis of targeted and untargeted lipidomics datasets to support biological interpretation.

Key Features:

• Input formats: Accepts mwTab files from Metabolomics Workbench, Skyline CSV Exports, and numerical matrices.
• Data types: Supports both untargeted and targeted lipidomics datasets generated by mass spectrometry.
• Preprocessing: Includes preprocessing steps for lipidomics datasets.
• Lipid name parsing: Automatically parses conventional lipid names to enable analyses by lipid class and chain length.
• Experimental variables: Accepts separate upload of experimental variables for tailored analyses.
• Exploratory analysis and subset selection: Enables exploratory data analysis and selection of subsets based on sample types or lipid characteristics.
• Statistical analyses: Performs univariate, multivariate, and unsupervised statistical methods.
• Confounder adjustment: Supports adjustment for confounding variables in complex experimental designs or clinical cohorts.
• Enrichment analysis: Performs lipid enrichment analysis for biological interpretation.

Scientific Applications:

• Differential lipidomics: Identification of differentially abundant lipids between conditions using targeted or untargeted data.
• Biological interpretation: Interpretation of lipidomic changes by lipid class and chain-length patterns and enrichment results.
• Complex experimental designs and clinical cohorts: Analysis of datasets requiring confounder adjustment and multivariate modeling.
• Re-analysis of existing datasets: Application to re-analysis of lipidomics data available in mwTab, Skyline CSV Exports, or matrix formats.

Methodology:

Accepts mwTab, Skyline CSV Exports, and numerical matrices; performs preprocessing; automatically parses conventional lipid names; conducts exploratory data analysis and subset selection; applies univariate, multivariate, and unsupervised statistical analyses; supports confounder adjustment; and performs enrichment analysis.