LiPlaCeT

LiPlaCeT performs 4D (3D + time) cell tracking and lineage analysis of live-imaging microscopy data within ImageJ to quantify cell dynamics such as position, length, growth rate, displacement, and proliferation in plant tissues including Arabidopsis thaliana roots.

Key Features:

• 4D Cell Tracking and Lineage Analysis: Enables tracking of cells in three spatial dimensions over time within plant tissues, with emphasis on primary and lateral root systems of Arabidopsis thaliana.
• Manual and Automated Tracking: Supports both manual tracking and automated tracking approaches to accommodate datasets with varying signal-to-noise ratios.
• ParaView Integration for Quantitative Analysis: Exports or interfaces tracking results to ParaView to derive quantitative metrics including cell length, position, growth rate, displacement rate, and proliferation activity at the tissue level.
• Genealogical and Clonal Analysis: Constructs genealogical trees and analyzes clonal relationships and cell dynamics in developing root primordia and the root apical meristem (RAM).

Scientific Applications:

• Root Morphogenesis and Proliferation Studies: Quantifies cell proliferation, growth, and spatial organization during development of primary and lateral roots in Arabidopsis thaliana.
• Lineage Reconstruction in Root Primordia: Reconstructs genealogical relationships to study clonal dynamics during root primordium formation.
• RAM Cell Dynamics Analysis: Investigates cell behavior and interactions in the root apical meristem over time.
• Population-level Quantitative Imaging: Enables tissue-scale analyses of displacement, growth rates, and proliferation across plant organs using live-imaging datasets.

Methodology:

LiPlaCeT couples advanced live-imaging microscopy data with ImageJ-based image analysis, supports manual and automated cell tracking, and integrates results with ParaView for automated quantitative analyses.