LOCATE

LOCATE catalogs membrane organization and subcellular localization data for proteins from the FANTOM3 Isoform Protein Sequence set in mouse, integrating MemO computational predictions with high-throughput immunofluorescence data and manual literature curation to provide experimentally verified localization and membrane-organization annotations covering approximately 40% of the mouse proteome.

Key Features:

• Curated annotations: Contains experimentally verified membrane organization and subcellular localization annotations for proteins derived from the FANTOM3 Isoform Protein Sequence set in mouse.
• MemO computational predictions: Uses the MemO pipeline to predict membrane organization at scale.
• High-throughput immunofluorescence: Includes subcellular localization data obtained from a high-throughput immunofluorescence-based assay.
• Manual literature curation: Incorporates results from an extensive manual review of over 1,700 peer-reviewed publications.
• Proteome coverage: Provides localization data covering approximately 40% of the mouse proteome.
• High-throughput methodology: Employs high-throughput approaches for systematic compilation of localization and membrane-organization data.

Scientific Applications:

• Subcellular localization studies: Enables analysis of protein distribution within cellular compartments and their membrane associations.
• Protein function and interaction analysis: Supports exploration of protein function and interaction networks in the context of cellular architecture.
• Cell and molecular biology research: Provides validated localization and membrane-organization data useful for studies in cell biology and molecular biology.

Methodology:

MemO computational pipeline is used to predict membrane organization at scale.