LookSeq

LookSeq visualizes aligned deep sequencing reads and represents paired-read evidence of structural variation to aid analysis of insertions, deletions, and genomic heterogeneity.

Key Features:

• Aligned-read visualization: Displays aligned sequence reads across genomic regions at multiple levels of resolution.
• Paired-read structural-variation depiction: Graphically represents paired sequence reads to reveal insertions, deletions, and other structural variants.
• Deep sequencing scalability: Handles large deep sequencing datasets and supports analysis of heterogeneous samples.

Scientific Applications:

• Structural variation analysis: Identification and inspection of insertions, deletions, and other variants supported by paired reads.
• Heterogeneity assessment: Analysis of within-sample and between-individual genomic heterogeneity in populations or heterogeneous samples.
• Complex genome studies: Examination of deep sequencing data from complex genomes to characterize genetic differences relevant to phenotype or disease susceptibility.

Methodology:

AJAX-based asynchronous data retrieval and on-demand rendering of aligned sequence reads for web-based visualization.