Life Science Knowledge Bank (LSKB)

Life Science Knowledge Bank (LSKB) aggregates transcriptomic, regulatory sequence, and functional annotation data to support comparative genomic analyses and drug-discovery research.

Key Features:

• Comparative Transcriptome Analysis: Identifies differential gene expression across conditions or species to elucidate conserved biochemical pathways.
• Gene Ontology Enrichment: Performs gene ontology enrichment to detect overrepresented biological processes, cellular components, and molecular functions in gene sets.
• Cis-regulatory Sequence Analysis: Analyzes cis-regulatory sequences to identify transcriptional regulators that control gene expression.
• Identification of Transcriptional Regulators: Detects regulators such as EP300, HDAC2, CEBPB, CEBPD, FOXA1, and FOXA2 implicated in adipocyte differentiation, lipid storage, and metabolism.
• Data Integration: Aggregates transcriptomic data from multiple sources to enable comprehensive analyses.
• Functional Annotation: Provides functional annotation of genes to support interpretation of expression and regulatory analyses.
• Cross-species Comparative Analysis: Facilitates comparisons between species (e.g., zebrafish and human) to identify conserved genetic mechanisms.

Scientific Applications:

• Elucidation of Therapeutic Mechanisms: Enables comparative analyses between models (for example zebrafish and humans) to reveal common therapeutic mechanisms such as effects observed with caloric restriction (CR) and resveratrol (RSV) in obesity studies.
• Identification of Key Transcriptional Regulators: Identifies regulators including EP300, HDAC2, CEBPB, CEBPD, FOXA1, and FOXA2 as targets for studying adiposity, lipid metabolism, and related pathways.
• Functional Analysis of Genetic Dysregulation: Analyzes genes dysregulated under specific conditions to provide insights into molecular bases of disease and potential therapeutic targets.

Methodology:

Data integration of transcriptomic datasets; computational algorithms for gene expression analysis, regulatory sequence identification, and functional annotation; and cross-species comparative studies.