M2Lite

M2Lite converts MSF files generated by Proteome Discoverer into mzIdentML to provide standardized representation of mass spectrometry-based proteomics identification results.

Key Features:

• MSF to mzIdentML conversion: Converts Proteome Discoverer MSF files into mzIdentML while preserving all relevant identification data.
• Standardization support: Produces mzIdentML output to enable interoperability and standardized representation of proteomics identification results.
• Lightweight and fast: Implemented for efficient, rapid conversion of MSF files.

Scientific Applications:

• Protein identification and peptide-spectrum assignment: Enables use of Proteome Discoverer search results in workflows for protein database searching and peptide-to-spectrum assignments by exporting to mzIdentML.
• Data integration and reproducibility: Facilitates sharing, comparison, and integration of proteomics identification results across tools and datasets to support reproducibility.

Methodology:

Takes MSF files as input and systematically converts them into the mzIdentML format while preserving all relevant data.