m6A-TCPred

m6A-TCPred predicts tissue-conserved N6-methyladenosine (m6A) sites at base resolution to distinguish conserved from non-conserved m6A modifications across 23 human tissues.

Key Features:

• Tissue profiling data: Leverages m6A profiling data derived from 23 human tissues.
• Feature integration: Integrates traditional sequence-based characteristics with additional genome-derived information.
• Base-resolution prediction: Produces predictions at base resolution to identify specific m6A residues.
• Conservation discrimination: Discerns distinct patterns between tissue-conserved and non-conserved m6A modifications.
• Performance metrics: Reports average AUROC of 0.871 in cross-validation and 0.879 on independent datasets.
• Annotated database: Includes 268,115 high-confidence m6A sites annotated with conserved status across 23 human tissues.

Scientific Applications:

• Tissue-specific epitranscriptomic studies: Enables analysis of m6A conservation patterns across multiple human tissues.
• Disease research: Supports investigation of m6A dysregulation in diseases, including cancer.
• Regulatory mechanism exploration: Assists in elucidating regulatory mechanisms and potential therapeutic targets associated with tissue-specific m6A marks.

Methodology:

Uses m6A profiling data from 23 human tissues and integrates sequence-based characteristics with genome-derived information; model performance was evaluated by cross-validation and independent datasets reporting AUROC values.