ma

ma provides an integrated digital atlas of mouse embryonic development combining Karl Theiler–staged 3D morphological models, curated image datasets, and spatially mapped in situ gene expression from the EMAGE database.

Key Features:

• 3D Modeling: Uses Karl Theiler's morphological staging system to generate detailed 3D models representing post-implantation stages of mouse development as a spatial framework for anatomical analysis.
• Gene Expression Data Integration: Archives and spatially maps in situ gene expression data within the EMAGE database across developmental stages and anatomical locations.
• IIP3D/X3DOM Viewer: Provides 3D visualization of anatomical structures and mapped gene expression patterns using IIP3D/X3DOM technologies.
• eHistology Resource: Supplies high-resolution histological sections for cellular-level examination of tissue architecture.
• Informatics Infrastructure: Employs informatics techniques to manage, curate, and enable retrieval and mining of image-based developmental biology datasets.

Scientific Applications:

• Spatial analysis of gene expression: Enables examination of where and when genes are expressed within the developing mouse embryo to inform gene function studies.
• Developmental anatomy mapping: Supports analysis of anatomical changes and morphology across Theiler-defined developmental stages.
• Investigation of morphological diversity: Facilitates comparison of anatomical and histological variation across stages or specimens.
• Study of genetic regulation: Provides spatial context to explore relationships between gene expression patterns and developmental processes.

Methodology:

Curation of image datasets structured according to Theiler's staging system; generation of 3D post-implantation models; integration of images with in situ gene expression from the EMAGE database; informatics-driven management and retrieval of image-based developmental biology datasets.