MALDIPepQuant

MALDIPepQuant quantifies peptides from Matrix-Assisted Laser Desorption/Ionization (MALDI) mass spectrometry using Stable Isotope Labeling by Amino acids in Cell culture (SILAC) to measure relative protein expression.

Key Features:

• Integration with SILAC: Implements SILAC-based peptide quantification by comparing labeled and unlabeled peptides.
• Compatibility with Phenyx output: Processes mass spectrometry data output from Phenyx for MALDI peptide quantification.
• Leucine labeling and sensitivity: Employs replacement of natural leucine with [(13)C6]leucine in a semidefined medium to enable quantification and detect at least 50% variation in protein expression.

Scientific Applications:

• Quantitative proteomics: Enables relative quantification of protein expression levels in proteomic studies.
• Microbial proteomics: Applied to organisms such as Bifidobacterium longum for peptide-level quantification.
• Biological investigations: Suitable for studies of cellular responses, disease mechanisms, and metabolic pathways that require peptide quantification.

Methodology:

Uses SILAC by replacing natural leucine with [(13)C6]leucine in a semidefined medium, differentiates labeled and unlabeled peptides during MALDI analysis, and quantifies peptides by analyzing Phenyx mass spectrometry output.