MAmBA

MAmBA maps 5-methylcytosine (m5C) and 5-hydroxymethylcytosine (hm5C) modifications in microRNAs using Bisulfite small RNA sequencing data for nucleotide-resolution epitranscriptomic profiling.

Key Features:

• High-Resolution Mapping: Employs BS-miRNA-seq, an NGS-based Bisulfite small RNA sequencing approach, to achieve precise site-level detection of (h)m5C in miRNAs.
• Comprehensive Analysis Pipeline: Uses the MAmBA analysis pipeline to process Bisulfite small RNA sequencing data and detect both CpG and non-CpG (h)m5C modifications across sequence contexts within miRNAs.
• Validation with Antibodies: Supports validation of detected m5C and hm5C residues using specific antibodies confirmed on mature human miRNAs.
• Broad Applicability: Applicable to Bisulfite small RNA sequencing datasets derived from diverse cell types and tissues.

Scientific Applications:

• Epitranscriptomic Research: Enables mapping of miRNA methylation landscapes to study post-transcriptional modification patterns in miRNAs.
• Biomarker Discovery: Facilitates identification of (h)m5C-modified miRNAs that may serve as disease-associated biomarkers.
• Functional Genomics: Supports investigation of how m5C and hm5C modifications influence miRNA stability, maturation, and target interactions.

Methodology:

MAmBA analyzes Bisulfite small RNA sequencing (BS-miRNA-seq) data with the MAmBA pipeline to detect and map CpG and non-CpG m5C and hm5C sites in miRNAs.