Mammalian Gene Collection

Mammalian Gene Collection provides full-length complementary DNA (cDNA) sequences and clones for human and mouse genes to support genomic and functional studies.

Key Features:

• Full-length cDNA and ORF generation: Generates and verifies full-length cDNA sequences and complete open reading frames (ORFs) for human and mouse genes.
• Specialized libraries: Creates specialized libraries enriched for full-length cDNAs used as sources for expressed sequence tags (ESTs).
• EST generation and analysis: Produces expressed sequence tags (ESTs) from enriched libraries and analyzes them to identify candidate clones containing complete ORFs.
• High-accuracy sequencing: Performs high-accuracy sequencing of candidate clones to verify complete ORFs.
• Database and repository development: Develops databases and clone repositories and distributes sequences and clones through public databases and clone distribution networks.
• Scale and coverage: Has sequenced and verified full ORFs for over 9,000 human genes and more than 6,000 mouse genes, and identified candidate full-ORF clones for approximately 7,800 human and 3,500 mouse genes.

Scientific Applications:

• Genetics: Provides verified full-length gene sequences and clones to support genetic research.
• Molecular biology: Supplies full-length cDNA clones for experimental molecular biology studies.
• Bioinformatics: Offers reference sequences to support bioinformatics analyses and gene annotation.
• Basic and applied biomedical studies: Supports basic research and applied biomedical investigations into mammalian genomics and implications for health and disease.

Methodology:

Analyzes expressed sequence tags (ESTs) derived from libraries enriched for full-length cDNAs to identify candidate clones with complete open reading frames (ORFs) and develops databases for sequence and clone metadata.