marker-free

The software tool joint method for marker-free alignment of tilt series in electron tomography (ET). The main features and advantages of this tool are:

1. It combines extrinsic (track-based) and intrinsic (intensity-based) alignment, making it the first joint solution.

2. The method utilizes the interrelationship between the projection model and the landmarks, allowing the localization of landmarks and projection parameters to refine each other iteratively until convergence.

3. The iterative approach makes the solution robust to different initial parameters and extreme geometric changes, ensuring better reconstruction for marker-free ET.

Topic

Tomography;Mapping

Detail

  • Operation: Read binning

  • Software interface: Command-line interface

  • Language: C++,C

  • License: Not stated

  • Cost: Free of charge

  • Version name: -

  • Credit: King Abdullah University of Science and Technology (KAUST), U.S. National Institutes of Health (NIH), Carnegie Mellon University's Center for Machine Learning and Health.

  • Input: -

  • Output: -

  • Contact: Min Xu mxu1@cs.cmu.edu ,Xin Gao xin.gao@kaust.edu.sa

  • Collection: -

  • Maturity: -

Publications

  • A joint method for marker-free alignment of tilt series in electron tomography.
  • Han R, et al. A joint method for marker-free alignment of tilt series in electron tomography. A joint method for marker-free alignment of tilt series in electron tomography. 2019; 35:i249-i259. doi: 10.1093/bioinformatics/btz323
  • https://doi.org/10.1093/BIOINFORMATICS/BTZ323
  • PMID: 31510669
  • PMC: PMC6612841

Download and documentation

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