mBARq

mBARq analyzes DNA barcodes from transposon insertion libraries, knockout mutants, and isogenic strain populations to enable fitness assessment across diverse experimental setups.

Key Features:

• Barcode Analysis: Processes DNA barcodes from random transposon mutagenesis screens, site-directed mutant attenuation studies, and isogenic strain pool population dynamics experiments.
• Statistical Analysis: Simplifies statistical analysis for diverse experimental setups involving barcode-based fitness data.
• Database Integration: Integrates with STRING and KEGG databases for interactive data exploration and contextualization.
• Web Application: Provides interactive data exploration through an intuitive web application interface.

Scientific Applications:

• Transposon Mutagenesis Screens: Assesses fitness of strains in random transposon mutagenesis screens through DNA barcode analysis.
• Knockout Mutant Analysis: Evaluates attenuation of site-directed knockout mutants using barcode-based approaches.
• Population Dynamics: Tracks population dynamics of isogenic strain pools via DNA barcoding.

Methodology:

The tool is implemented in Python.