Microbial Genome Database (MBGD)

Microbial Genome Database (MBGD) provides automated identification and comparative analysis of orthologous genes across microbial genomes to support studies of genome diversity, function, and evolution.

Key Features:

• Automated Ortholog Group Identification: Automatic identification of orthologous genes across a wide array of microbial genomes.
• Customizable Ortholog Groups: Creation of ortholog groups tailored to user-specified subgroups of organisms for targeted comparative analyses.
• Comprehensive Database: A continually updated repository encompassing nearly 1,000 genomes.
• Enhanced Functional Annotation: Assignment of functional annotations to orthologous groups with links to external databases.
• Phenotype-Driven Genome Comparison: Support for selecting genomes for comparison based on phenotypic properties.
• Inclusion of Eukaryotic Microbial Genomes: Coverage includes eukaryotic microbes such as fungi and protists and some higher eukaryotes in addition to prokaryotes.
• MyMBGD Mode: Acceptance of raw genomic sequences without prior annotation, with automatic execution of a gene-finding procedure before ortholog identification.
• Improved Analysis Functions: Functions to identify orthologs with similar phylogenetic patterns for evolutionary analysis.

Scientific Applications:

• Comparative Genomics: Cross-genome ortholog identification for comparative analyses among bacteria, archaea, fungi, protists, and selected higher eukaryotes.
• Genome Diversity Studies: Characterization of microbial genome diversity using ortholog group composition across nearly 1,000 genomes.
• Functional Annotation and Evolution: Linking orthologous groups to functional annotations and external databases to infer gene function and evolutionary relationships.
• Phenotype-Driven Genomic Studies: Comparative analyses that correlate phenotypic properties with genomic content across selected genomes.
• Novel Genome Integration: Incorporation and analysis of raw genomic sequences via MyMBGD by performing gene finding prior to ortholog assignment.
• Phylogenetic Pattern Analysis: Identification of orthologs with conserved phylogenetic patterns to support evolutionary inference.

Methodology:

Automated ortholog-group identification; creation of subgroup-specific ortholog groups; automatic gene-finding on raw genomic sequences prior to ortholog assignment (MyMBGD); assignment of functional annotations with links to external databases; identification of orthologs with similar phylogenetic patterns.