mblastall

mblastall performs nucleotide database searches using nucleotide query sequences to identify homologous sequences for gene annotation and evolutionary and functional analysis.

Key Features:

• Database Search Capability: Searches nucleotide query sequences against nucleotide databases and performs sequence alignment and comparison to detect similarity.
• Integration with Galaxy Platform: Integrated with the Galaxy platform (Afgan et al., 2016) and the Galaxy@Pasteur instance for workflow execution and interoperability.
• Execution Engine: Functions as an execution engine leveraging Institut Pasteur cluster resources and communicating via the Galaxy API and the Bioblend library.

Scientific Applications:

• Metagenomic analysis (MetaGenSense): Supports workflows such as MetaGenSense for metagenomic data analysis launched on the Galaxy platform.
• Phylogenetic studies (NGphylogeny.fr): Facilitates nucleotide sequence comparisons used by NGphylogeny.fr to infer evolutionary relationships.
• Metagenomic analysis (Shaman): Aids applications like Shaman in interpreting complex microbial community sequence data.

Methodology:

Employs sequence alignment algorithms to compare query sequences against database entries and identify regions of similarity, and uses the Galaxy API and Bioblend library to communicate with web services and the Galaxy instance while leveraging Institut Pasteur cluster resources.