MeDAS

MeDAS provides comprehensive quantitative data on developmental alternative splicing by re-analyzing Illumina polyA+ RNA-seq time-course libraries across metazoans to support comparative and developmental splicing analyses.

Key Features:

• Extensive data repository: Re-analysis of a curated collection of 2,232 Illumina polyA+ RNA-seq libraries to produce quantitative measurements of alternative splicing events.
• Broad taxonomic and temporal scope: Time-course data covering embryonic and post-natal development in 18 species, from Caenorhabditis elegans to humans, representing major metazoan lineages.
• Multilevel splicing visualization: Visualization of alternative splicing at gene, transcript, and exon levels with time-course expression profiles and splicing inclusion levels.

Scientific Applications:

• Temporal regulation of splicing: Investigating how alternative splicing patterns change across developmental time courses.
• Isoform function in development: Identifying trends and potential functional roles of transcript isoforms during embryonic and post-natal development.
• Comparative and evolutionary analyses: Assessing conserved and divergent splicing mechanisms across metazoan species.

Methodology:

Re-analysis of a curated collection of 2,232 Illumina polyA+ RNA-seq libraries to quantify alternative splicing events and inclusion levels across developmental time courses in 18 metazoan species.