mesoSPIM initiative

mesoSPIM initiative enables volumetric light-sheet microscopy of centimeter-scale cleared and expanded biological samples, providing near-isotropic resolution with rapid acquisition for large-scale tissue imaging.

Key Features:

• Open-Hardware Platform: Provides open-source hardware designs for constructing customizable light-sheet microscopes.
• Compatibility: Accommodates cleared and expanded samples of various types and sizes, including centimeter-scale specimens.
• High Resolution and Speed: Delivers near-isotropic spatial resolution and rapid volumetric acquisition to image large samples within minutes.

Scientific Applications:

• Large-Scale Imaging: Enables mesoscale imaging of whole organs and large tissue sections for volumetric anatomical and structural studies.
• Versatile Sample Compatibility: Supports imaging of diverse cleared and expanded sample preparations for comparative and multi-specimen experiments.

Methodology:

Employs light-sheet microscopy to perform volumetric imaging of cleared and expanded samples, reducing photodamage while enabling near-isotropic resolution and rapid capture of centimeter-scale specimens.