MiCroKit

MiCroKit catalogs experimentally validated proteins localized to the midbody, centrosome, and kinetochore to support studies of cell division fidelity.

Key Features:

• Comprehensive Protein Collection: Contains 1,489 microkit proteins from seven model organisms—Saccharomyces cerevisiae, Schizosaccharomyces pombe, Caenorhabditis elegans, Drosophila melanogaster, Xenopus laevis, Mus musculus, and Homo sapiens—each validated for subcellular localization by fluorescent microscopy.
• Orthologous Information: Provides orthologous relationships for each microkit protein to enable cross-species comparison and identification.

Scientific Applications:

• Cell Biology Research: Supports analysis of spatial and temporal assembly of protein complexes during mitosis by supplying localization-validated protein data.
• Comparative Genomics: Enables exploration of evolutionary conservation and functional inference via ortholog comparisons across the seven model organisms.
• Experimental Design: Assists identification and validation of candidate proteins involved in cell division by referencing experimentally localized microkit proteins.

Methodology:

Manually curated from scientific literature, including only proteins with unambiguous experimental evidence of subcellular localization (fluorescent microscopy).