Micropilot

Micropilot automates detection of cells of interest and the initiation of complex microscopy experiments such as three-dimensional multicolor time-lapse imaging and fluorescence recovery after photobleaching (FRAP) in live cells to support quantitative microscopy and systems biology.


Key Features:

  • Automated Cell Detection: Identifies cells of interest within large populations for targeted imaging.
  • Automated Initiation of Complex Imaging Experiments: Autonomously launches three-dimensional multicolor time-lapse imaging and fluorescence recovery after photobleaching (FRAP) in live cells upon detection of relevant targets.
  • Adaptive Learning Algorithms: Uses advanced algorithms to learn from experimental setups and recognize cells exhibiting specific features.
  • Autonomous Imaging Protocol Management: Manages and executes complex imaging protocols without manual intervention.

Scientific Applications:

  • Systems Biology Research: Enables detailed statistical analysis of cellular processes to support systems biology studies.
  • Enhanced Experimental Efficiency: Increases throughput and reduces time for complex microscopy experiments by automating cell selection and experiment initiation.

Methodology:

Micropilot leverages advanced algorithms to learn from experimental setups and adaptively recognize cells exhibiting specific features, and it autonomously manages complex imaging protocols.

Topics

Details

Tool Type:
command-line tool
Operating Systems:
Windows
Added:
12/18/2017
Last Updated:
11/25/2024

Operations

Publications

Conrad C, Wünsche A, Tan TH, Bulkescher J, Sieckmann F, Verissimo F, Edelstein A, Walter T, Liebel U, Pepperkok R, Ellenberg J. Micropilot: automation of fluorescence microscopy–based imaging for systems biology. Nature Methods. 2011;8(3):246-249. doi:10.1038/nmeth.1558. PMID:21258339. PMCID:PMC3086017.

Links