miFRame

miFRame analyzes human small RNA next-generation sequencing (NGS) data to identify and quantify known mature microRNAs and to predict novel miRNA precursors and iso-microRNAs for biomarker discovery.

Key Features:

• Comprehensive Data Analysis: Supports detailed examination of human small RNA NGS data, accommodating known mature microRNAs and predicted novel sequences derived from known precursors.
• Expression Analysis: Quantifies expression for precursor molecules and their corresponding mature miRNAs.
• Novel Discovery Capabilities: Detects novel precursors and potential iso-microRNAs to expand identifiable miRNA variants.
• Result Aggregation and Validation: Aggregates results across users to evaluate reproducibility of novel mature miRNAs and to distinguish experiment-specific findings from consistent observations.

Scientific Applications:

• Biomarker Discovery: Applied to circulating biomarker screening in Multiple Sclerosis (MS) and Alzheimer's Disease using cohorts with disease stages and matched controls to filter likely false positives.
• Clinical Relevance: Identifies disease-associated miRNA expression patterns to support diagnostic and translational research.

Methodology:

Processes and interprets NGS small RNA data using computational techniques and integrates analytical modules.