MINRbase

MINRbase provides access to 130,238 expressed rRNA fragments (rRFs) derived from human nuclear rRNAs (18S, 5.8S, 28S, 5S), mitochondrial rRNAs (12S, 16S), and internal spacers of the 45S pre-rRNA for comparative analysis of rRF expression across tissues, disease states, sex, and ancestry.

Key Features:

• Database content: Contains 130,238 expressed rRFs mapped to human nuclear rRNAs (18S, 5.8S, 28S, 5S), mitochondrial rRNAs (12S, 16S), and internal spacers of the 45S pre-rRNA.
• Data provenance: Compiled from analysis of 11,632 datasets from GEUVADIS and The Cancer Genome Atlas (TCGA).
• Query capabilities: Supports queries by parental rRNA identity, nucleotide sequence, abundance levels, and metadata including tissue type, disease, sex, and ancestry.
• Expression profiles: Provides per-rRF summary profiles reporting expression across variables such as tissue type, disease state, sex, and ancestry.
• Visualization output: Produces publication-ready plots for visualization and interpretation of rRF expression data.

Scientific Applications:

• Expression profiling: Comparative analysis of rRF abundance across tissues and disease states.
• Origin characterization: Identification and characterization of rRFs derived from 45S internal spacers and rRFs that extend beyond annotated rRNA boundaries.
• Association studies: Investigation of associations between rRF production and personal attributes (sex, ancestry) and contextual factors (tissue type, state, disease).
• Genomics and molecular biology research: Resource for studies on rRNA processing, small RNA biology, and rRF functional hypotheses.

Methodology:

Analysis of 11,632 datasets from GEUVADIS and The Cancer Genome Atlas (TCGA) was used to identify and quantify 130,238 expressed rRFs.