MIPModDB

MIPModDB catalogs comparative models and annotated sequence features of major intrinsic proteins (MIPs) to support analysis of their transport functions for water, glycerol, other neutral molecules, and gases.

Key Features:

• Extensive Data Collection: MIPModDB contains information on over 1000 identified major intrinsic proteins (MIPs) compiled from analysis of publicly available genome sequences.
• Detailed Entry Annotations: Each entry annotates the source organism, gene structure and sequence features, substitutions within conserved NPA motifs, structural models, residues forming the selectivity filter, and channel radius profile.
• Structure-Based Analysis: Provides structure-based sequence alignments for selected sets of MIPs to facilitate comparative and evolutionary studies.
• Downloadable Sequences and Models: Offers downloadable sequences and structural models of selected MIPs for downstream analysis.

Scientific Applications:

• Comparative Genomics: Elucidating evolutionary relationships among MIPs across different species.
• Protein Engineering: Designing MIP variants with altered transport properties for biotechnological applications.
• Biophysics and Structural Biology: Investigating the structural determinants of selectivity and permeability in membrane channels.

Methodology:

The database was built by systematic analysis of genome sequences to identify and catalog MIPs, followed by detailed annotation of sequence features and structural models.