miRAGDB

miRAGDB catalogs miRNAs and integrates NGS and mRNA expression data to characterize regulation of RAG1 and RAG2 during B‑ and T‑cell development and in blood cancers.

Key Features:

• miRNA Cataloging: Identifies and catalogs miRNAs that regulate RAG1 expression in normal development and cancer.
• Comprehensive Data Integration: Integrates next-generation sequencing (NGS) data from human and mouse models across developmental stages of B-cells and T-cells and from blood cancer samples.
• Expression Analysis: Provides expression profiles of miRNAs targeting RAG1, reporting 1,173 miRNAs in humans and 749 in mice, and includes mRNA expression data for ten transcription factors and interacting partners involved in RAG regulation.
• Visualization and Analysis: Enables plotting and analysis of expression patterns of RAG regulators across different biological contexts.

Scientific Applications:

• Developmental Biology: Investigates miRNA-mediated regulation of RAG expression during early B-cell and T-cell development.
• Oncology Research: Examines aberrant RAG regulation in leukemia and lymphoma to inform studies of oncogenic translocations and molecular mechanisms in blood cancers.
• Gene Regulation Studies: Supports analysis of post-transcriptional gene regulation mediated by miRNAs targeting RAG genes and their interacting partners.

Methodology:

Leverages NGS data to analyze miRNA expression against RAG1 in normal and diseased states and organizes these miRNA profiles alongside mRNA expression profiles of key transcription factors and interacting partners.