MiRaGE

MiRaGE infers miRNA-mediated regulation of target genes from changes in gene expression profiles.

Key Features:

• Inference of miRNA Regulation: MiRaGE deduces miRNA regulation by analyzing shifts in gene expression data.
• Normalization-free Analysis: The method uses gene expression changes without requiring prior array normalization.
• Ranking System: MiRaGE ranks miRNAs by inferred regulatory impact on gene expression profiles.

Scientific Applications:

• Cell growth, differentiation and development: Used to investigate miRNA-mediated regulation in processes of cell growth, differentiation and development.
• Embryonic stem (ES) cell differentiation into neuronal cells: Applied to ES cell differentiation to identify miRNAs implicated in maintaining the undifferentiated state during neuronal differentiation.
• miRNA candidate identification: Identified candidate miRNAs such as the miR-200 family, miR-429, the miR-302 family, and members of the miR-17-92 cluster as potentially pivotal in ES cell pluripotency.

Methodology:

Analyzes changes in gene expression profiles to infer miRNA activity without requiring prior array normalization and ranks miRNAs by inferred regulatory impact.