miREV

miREV identifies potential reference microRNAs (miRNAs) in small-RNA sequencing datasets from extracellular vesicles (EVs) to support selection of reference RNAs for NGS validation and RT-qPCR.

Key Features:

• Reference miRNA identification: Identifies candidate reference miRNAs within small-RNA sequencing datasets derived from extracellular vesicles (EVs).
• Dataset collection: Comprises over 400 miRNA sequencing datasets for use in comparative analyses.
• Sample set assembly: Assembles sample sets analogous to a user's experimental design to tailor reference selection to study-specific conditions.
• Data export: Exports raw read count tables and associated metadata from generated sample sets.
• Validation support: Provides reference candidates intended for validation of NGS experiments and normalization in RT-qPCR.
• Biomarker relevance: Supports identification of EV miRNAs relevant to biomarker studies and liquid biopsy applications.

Scientific Applications:

• Reference selection for validation: Selection of stable reference RNAs for validating small-RNA NGS results and RT-qPCR.
• Normalization of expression analyses: Generation of study-specific reference panels for normalization in EV miRNA expression studies.
• Biomarker discovery and validation: Prioritization of EV miRNA candidates for biomarker studies using liquid biopsies.

Methodology:

Assembles sample sets from the repository of over 400 miRNA sequencing datasets, identifies candidate reference miRNAs from small-RNA sequencing data, and outputs raw read count tables with associated metadata.