MiRNAkey

MiRNAkey analyzes miRNA deep-sequencing (NGS) data to quantify miRNA expression and report differential expression in paired samples.

Key Features:

• Pipeline implementation: Implements common computational steps required for miRNA deep-sequencing analysis.
• Data statistics and multiple read determination: Provides comprehensive statistical analysis of sequence reads and supports multiple read determination.
• Detailed reporting: Generates tabular and graphical reports on sequence reads, including general and detailed summaries and identification of differentially expressed miRNAs in paired samples.

Scientific Applications:

• Gene expression studies: Quantifies miRNA expression profiles to support investigations of post-transcriptional regulation of gene expression.
• Disease research: Identifies differentially expressed miRNAs relevant to diseases such as cancer, cardiovascular disorders, and neurological conditions.
• Developmental biology: Profiles changes in miRNA expression during development to study roles in cellular differentiation and growth.

Methodology:

Performs statistical analysis of sequence reads, multiple read determination, generates tabular and graphical outputs, and identifies differentially expressed miRNAs in paired samples.