mMCP-counter

mMCP-counter quantifies murine immune and stromal cell populations from transcriptomic data to characterize tissue cellular composition and inform preclinical studies.

Key Features:

• Specificity and Accuracy: Employs highly specific transcriptomic markers to quantify 12 distinct immune cell populations and 4 stromal cell types in murine tissues.
• Validation and Performance: Validated using flow cytometry data, with comparative analyses demonstrating that it outperforms existing methods for mouse studies.
• Predictive Capability: Produces quantification scores that are predictive of response to immune checkpoint blockade therapies in mouse models of mesothelioma and kidney cancer.
• Broad Application Potential: Applied beyond oncology, including analyses of immune impacts in Alzheimer's disease.

Scientific Applications:

• Cancer Research: Provides immune and stromal composition profiles of tumors to study tumor–immune interactions and support immunotherapy research.
• Immunology: Enables analysis of immune cell population dynamics and immune responses across disease contexts in murine models.
• Neurodegenerative Disease Research: Supports investigation of neuroinflammation and the roles of immune cells in Alzheimer's disease models.

Methodology:

Leverages transcriptomic data and a curated set of gene expression markers specific to murine immune and stromal cells to estimate cell population abundances; implemented as an R package.