MMTB

MMTB extends the Metano toolbox to construct and analyze genome-scale metabolic models, enabling metabolite-centric and reaction-centric analyses of metabolic flux distributions.

Key Features:

• Metabolite-centric analysis: Implements Metano's split-ratio analysis and metabolite flux minimization to quantify flux distribution at the metabolite level.
• Reaction-centric analysis: Supports flux balance analysis (FBA) for reaction-centric examination of steady-state flux distributions.
• Model construction and annotation: Supports bottom-up construction of genome-scale metabolic models with integration of reaction and enzymatic annotation data from external databases.
• Genome-scale model support: Operates on genome-scale metabolic models for analysis and refinement.

Scientific Applications:

• Systems biology: Enables analysis and prediction of steady-state metabolic behavior and flux distributions in genome-scale models.
• Synthetic biology: Supports evaluation of metabolic engineering strategies through metabolite- and reaction-centric flux analyses.
• Microbial ecology: Facilitates analysis of metabolic network responses to varying environmental conditions in microbial systems.
• Model development and validation: Demonstrated by analysis of a published Corynebacterium glutamicum model and the construction of a new Phaeobacter inhibens model.

Methodology:

Integration of Metano's analytical methods with computational support for flux balance analysis (FBA), split-ratio analysis, metabolite flux minimization, and bottom-up model construction using reaction and enzymatic annotation data from databases.