Mobi

Mobi defines binary structural mobility in NMR-derived protein ensembles to identify flexible regions relevant to intrinsic disorder characterization and comparative modeling.

Key Features:

• Binary Mobility Definition: Uses structural superposition and local conformational difference analysis to produce a binary classification of mobile versus rigid residues that aligns with manually curated intrinsic disorder definitions from CASP8.
• Output Formats: Generates mobility-colored PDB files for structural inspection, mobility plots for graphical representation of mobile regions, and a FASTA-formatted sequence file summarizing mobility assignments.

Scientific Applications:

• Structural Analysis: Enables identification and mapping of flexible regions in NMR structures to inform studies of protein dynamics and function.
• Comparative Modeling: Defines mobile regions to improve reliability of comparative modeling and protein structure prediction workflows.
• Intrinsic Disorder Studies: Supports analyses exploring relationships between structural mobility and intrinsic disorder by providing mobility definitions analogous to disorder annotations.

Methodology:

Structural superposition of NMR ensembles and analysis of local conformational differences are used to identify regions with significant mobility.