MODEST

MODEST designs oligonucleotides for MAGE and recombineering to generate targeted genomic modifications and combinatorial variant libraries.

Key Features:

• Comprehensive design capabilities: Designs oligos to tune translation rates by modifying ribosomal binding sites, generate translational gene knockouts, and introduce amino acid substitutions, insertions, deletions, and point mutations.
• Automation: Automates conversion of user-defined genotypic or phenotypic specifications into oligonucleotide sequences for MAGE and recombineering.
• High efficiency in genome engineering: Supports creation of single mutants and combinatorial cell libraries to enable high-efficiency generation of genetic variants.

Scientific Applications:

• Synthetic biology: Enables modification of genomic or plasmid sites to tune protein expression levels and construct engineered strains.
• Functional genomics: Enables targeted mutations and translational knockouts to investigate gene function.
• Metabolic engineering: Facilitates combinatorial variant generation to optimize metabolic pathways in microorganisms.

Methodology:

Computational algorithms translate user-defined genotypic or phenotypic specifications into precise oligonucleotide sequences for MAGE and recombineering.