Mol Biology Tools
Molecular Biology Tools is a free browser-based collection of molecular biology utilities for routine sequence analysis, primer design, Sanger sequencing primer planning, cloning setup, and wet-lab calculations. The site includes tools for PCR primer design, Sanger primer design and primer walking, primer binding checks, restriction site analysis, reverse complement generation, ORF and protein translation, codon optimization, ligation calculations, molarity calculations, dilution calculations, and multi-solute solution recipe preparation. The tools run in the browser and are intended for quick experimental planning, without requiring logins or uploading sequences to the server.
Topics
Details
- License:
- Proprietary
- Maturity:
- Emerging
- Cost:
- Free of charge
- Tool Type:
- api
- Operating Systems:
- Android, Linux, Mac, Windows, iOS
- Programming Languages:
- JavaScript
- Added:
- 6/6/2026
- Last Updated:
- 7/19/2026
Operations
Documentation
Quick start guide
https://molbiologytools.com/help_guidesLinks
Service
https://molbiologytools.com/buffer_calculator(A free online tool for preparing common lab buffer recipes by target pH, buffer strength, salt form, and final volume.)
Service
https://molbiologytools.com/codon_optimizer(Codon Optimizer is a free online optimization tool for preserving the translated protein sequence while checking GC content, codon changes, and downstream primer design.)
Service
https://molbiologytools.com/ligation_calculator(DNA ligation calculator for cloning setup, vector mass, insert length, insert-to-vector molar ratio, and insert mass calculation.)
Service
https://molbiologytools.com/orf_protein_translator(Free online ORF finder and DNA to protein translator for open reading frames, coding-region checks, amino acid translation, and expression planning.)
Service
https://molbiologytools.com/sanger_primer_designer(Free online Sanger sequencing primer design tool for primer walking, target coverage, high-quality read planning, and binding validation.)
Service
https://molbiologytools.com/dilution_calculator(Free online dilution calculator for C1V1 logic, prepared stock solutions, and antibody 1:N ratios; molecular weight is required only for cross-unit conversion.)
Service
https://molbiologytools.com/reverse_complement(Generate a reverse complement, complement, reversed sequence, or cleaned DNA from plain-text, FASTA, or GenBank input, with full IUPAC ambiguity-code support)
Service
https://molbiologytools.com/primer_design(PCR primer design tool for raw DNA, FASTA, or GenBank templates, with primer length or target Tm modes and optional restriction-enzyme overhang planning.)
Service
https://molbiologytools.com/primer_binding_checker(Primer binding checker for linear or circular DNA templates, including binding positions, amplicon size, strand direction, and product checks.)
Service
https://molbiologytools.com/restriction_site_analyzer(Restriction enzyme site analyzer for linear or circular DNA, FASTA, or GenBank input, with enzyme cut-site detection and sequence-to-primer workflow links.)
Other
https://molbiologytools.com/faq(Searchable FAQ covering primer design, Sanger sequencing, restriction sites, sequence checks, molarity, dilution, ligation, and connected tool workflows.)
Service
https://molbiologytools.com/molarity_calculator(This free online molarity calculator handles single-solute mass, molarity, final-volume and g/L calculations, liquid acid stocks, and protein molarity conversions using molecular weight in kDa)