MonoRes

MonoRes computes local resolution in three-dimensional electron microscopy (EM) maps using the monogenic signal framework to assess resolution at each voxel.

Key Features:

• Monogenic signal framework: Extends the analytic signal concept by employing the monogenic signal to analyze local amplitudes in 3D EM maps.
• Automatic operation: Operates autonomously without requiring user-defined parameters.
• Frequency filtering: Filters the 3D EM map at different frequencies to obtain scale-dependent information.
• Amplitude-based voxel-wise criterion: Computes the amplitude of the monogenic signal across frequencies and applies an amplitude-based criterion to determine resolution at each voxel.
• Local filtering option: Optionally applies local filtering of the original map using the computed local resolution values.
• Computational efficiency: Performs resolution estimation more quickly than existing methods.
• High accuracy: Demonstrates high accuracy in determining local resolution across validation tests.

Scientific Applications:

• Model building and validation: Provides voxel-wise resolution estimates to guide model building and validation from 3D EM maps.
• Interpretation of molecular structures: Reveals spatial variability of resolution within maps to aid interpretation of molecular features at different scales.

Methodology:

Filter the 3D EM map at different frequencies, compute the amplitude of the monogenic signal at each frequency, apply an amplitude-based criterion to assign a resolution value to each voxel, and optionally locally filter the original map using the computed local resolution values.