Mouse GPCRs and Human and Rat orthologs

Mouse GPCRs and Human and Rat orthologs maps GPCR expression across mouse renal tubule segments and provides human and rat ortholog annotations to support cross-species comparative analysis using RNA sequencing and protein mass spectrometry data.

Key Features:

• Comprehensive Database: Curates a list of G protein-coupled receptors (GPCRs) across mouse, rat, and human genomes from multiple online genomic databases.
• Segment-Specific Expression Data: Identifies GPCR expression patterns using RNA-sequencing data from microdissected mouse renal tubule segments.
• Cross-Species Orthologs: Maps human and rat orthologs to mouse GPCRs to enable comparative analyses and translational interpretation.
• Integration with Omics Data: Integrates GPCR expression with protein mass spectrometry and other omics datasets and with functional datasets such as isolated perfused tubule and micropuncture experiments.
• Research Applications: Highlights well-characterized and understudied GPCRs to inform studies of kidney transport mechanisms, renal physiology, and disease.

Scientific Applications:

• Renal segment-specific receptor identification: Pinpoints receptors selectively expressed in specific renal tubule segments for functional hypothesis generation.
• Cross-species translational mapping: Enables translation of mouse expression data to human and rat via ortholog annotations.
• Validation with functional datasets: Supports comparison and validation of expression patterns against isolated perfused tubule and micropuncture experiment data.
• Prioritization of understudied GPCRs: Facilitates identification and prioritization of poorly studied GPCRs for experimental follow-up in renal transport and disease research.

Methodology:

Curates GPCR lists from existing genomic databases; mines RNA-sequencing data from microdissected mouse tubule segments and incorporates protein mass spectrometry datasets; performs comparative analyses with functional datasets including isolated perfused tubule and micropuncture experiments.