Mousepost

Mousepost catalogs protein-coding sequence polymorphisms across inbred mouse strains to support comparative genetic analysis and identification of variant effects on protein function and phenotype.

Key Features:

• Expanded Strain Coverage: Includes protein-coding sequences from the original 36 sequenced inbred strains plus 16 additional strains (total 52) referenced to the C57BL/6J genome using data from the Mouse Genomes Project.
• Comprehensive Polymorphism Analysis: Searches and catalogs variations in all protein-coding gene sequences across included strains and categorizes sequence polymorphisms that may influence strain-specific phenotypes or act as modifier effects.
• Enhanced Mutation Detection: Detects and classifies start codon mutations in addition to stop codon losses and gains, small insertions and deletions (indels), and missense mutations.

Scientific Applications:

• Comparative genomics: Enables detailed comparisons of protein-coding sequences across multiple mouse strains to study genetic diversity.
• Phenotype and disease susceptibility studies: Facilitates identification of variants that contribute to phenotypic diversity and disease susceptibility.
• Genetic modifier discovery: Supports identification of potential genetic modifiers that may influence experimental outcomes or strain-specific traits.

Methodology:

Performs systematic comparison of protein-coding gene sequences from multiple mouse strains against the C57BL/6J reference genome and catalogs and analyzes sequence polymorphisms, including start codon mutations, stop codon losses and gains, small insertions and deletions, and missense variants.