MPRAbase

MPRAbase provides a manually curated repository for examining and downloading Massively Parallel Reporter Assay (MPRA) data across cell types and organisms.

Key Features:

• Comprehensive Data Collection: The database currently harbors 130 experiments encompassing 17,718,677 elements tested across 35 cell types and 4 organisms.
• Cross-Species and Cross-Cell-Type Comparison: Users can examine the activity of regulatory elements across different cell types and organisms.
• Data Download: MPRA data is available for independent analysis and download.

Scientific Applications:

• Regulatory Element Discovery: MPRAbase supports identification of functional regulatory sequences from MPRA experiments.
• Variant Effect Measurement: The database enables analysis of how genetic variants affect gene regulatory activity.
• Saturation Mutagenesis Studies: Users can access comprehensive mutagenesis data to understand sequence-function relationships.
• Synthetic Regulatory Element Characterization: The repository includes data on engineered regulatory sequences.
• Evolutionary Gene Regulatory Analysis: MPRAbase facilitates comparison of gene regulatory differences across species.

Methodology:

MPRAbase employs manual curation to collect and integrate MPRA experimental results from multiple studies.