MPTherm

MPTherm aggregates thermodynamic measurements and annotations for membrane proteins to support analysis of protein stability, including melting temperature, free energy, and enthalpy.

Key Features:

• Extensive Data Collection: Contains over 7,000 thermodynamic data points from approximately 320 membrane proteins collated from the literature and ProThermDB.
• Detailed Annotation: Each entry includes protein sequence, structural details, membrane topology, experimental conditions, melting temperature, free energy, enthalpy, and precise literature references.
• Cross-Linking with Major Databases: Entries are cross-referenced to UniProt and PDB to connect sequence and structural information with thermodynamic data.
• Visualization Tools: Provides sequence and structure visualization capabilities to interpret thermodynamic parameters in a structural context.

Scientific Applications:

• Understanding Stability: Facilitates analysis of how structural features and membrane topology correlate with membrane protein stability and thermodynamic parameters.
• Predictive Model Development: Serves as a dataset for developing predictive models and tools to forecast membrane protein behavior under varying experimental conditions.
• Drug Target Characterization: Supports identification of mutations or structural features associated with altered stability or function relevant to membrane protein drug targets.

Methodology:

Manual curation and integration of thermodynamic data from existing literature and databases with annotation of experimental conditions and membrane topology details.