mres2x

mres2x converts Mascot MIME result files into database-compatible tabular formats to enable SQL-based storage and querying of MS and MS/MS (shotgun proteomics) datasets and to extract original DTA peak lists for Sequest.

Key Features:

• Format Conversion: Converts Mascot result files from MIME format into tabular formats suitable for import into relational database management systems (RDBMS).
• Output Flexibility: Produces tabular outputs optimized for RDBMS import and extracts original peak lists in DTA format compatible with Sequest.
• Data Management: Enables regrouping of MS/MS results and analysis via SQL queries without requiring new Mascot searches when changing grouping parameters.
• Consistency Checks: Performs consistency checks during conversion to ensure data integrity and accuracy.
• High Throughput Processing: Supports high-throughput processing and integration with other computational workflows.

Scientific Applications:

• Protein Identification: Facilitates integration of Mascot results with Sequest by extracting DTA peak lists for further protein identification.
• Data Analysis: Enables complex querying and large-scale analyses of MS and MS/MS data via RDBMS and SQL for proteomics studies.
• Workflow Efficiency: Reduces redundant Mascot searches when modifying analysis parameters by allowing data regrouping and reanalysis from the database.

Methodology:

Converts Mascot MIME result files into database-compatible tabular formats and generates tab files optimized for RDBMS import; extracts original peak lists in DTA format; performs consistency checks during conversion and produces outputs that enable regrouping of MS/MS results and SQL-based analyses while supporting high-throughput integration.