mRNArchitect

mRNArchitect assists in the design of mRNA sequences by rapidly assembling and optimizing mRNA primary sequences based on user requirements for GC content, secondary structure, codon optimization, and uridine depletion.

Key Features:

• Sequence Assembly: Assembles mRNA primary sequences from multiple elements including the coding sequence of the target protein or gene, flanking untranslated regions, and the poly(A) tail.
• GC Content Optimization: Optimizes mRNA sequences based on GC content requirements.
• Secondary Structure Optimization: Optimizes mRNA sequences based on secondary structure requirements.
• Codon Optimization: Optimizes mRNA sequences based on codon optimization requirements.
• Uridine Depletion: Optimizes mRNA sequences based on uridine depletion requirements.
• Experimental Validation: Generates sequences that can be readily synthesized and have been experimentally validated across a variety of applications.

Scientific Applications:

• mRNA Medicine Design: Assists scientists in designing new mRNA medicines by optimizing sequences to increase translation and stability, and reduce reactogenicity.
• Protein Expression Engineering: Supports sequence design incorporating coding sequences of target proteins or genes with optimized expression through flanking untranslated regions.

Methodology:

mRNArchitect employs computational sequence assembly and optimization algorithms to generate mRNA sequences meeting specified requirements for GC content, secondary structure, codon usage, and uridine content.