Mslar

Mslar catalogs curated and predicted synthetic lethal (SL) and synthetic rescue (SR) gene interactions in microbial genomes to support genetic interaction research.

Key Features:

• Curated experimental SL pairs: Contains 13,313 experimentally validated synthetic lethal gene pairs extracted from the literature.
• Curated experimental SR pairs: Contains 2,994 experimentally validated synthetic rescue gene pairs extracted from the literature.
• Putative SL predictions by homologous transfer: Includes 86,981 putative SL pairs inferred via homologous transfer across 281 bacterial genomes.
• Sequence similarity searches (BLAST): Provides BLAST-based sequence searches for identifying homologs and mapping relationships relevant to SL/SR pairs.
• Saccharomyces cerevisiae SL analysis: Includes an analysis examining essentiality of duplicated genes versus singletons in SL contexts for S. cerevisiae.

Scientific Applications:

• Microbial genetic interaction mapping: Supports investigation and cataloging of synthetic lethality and synthetic rescue relationships within microbial systems.
• Comparative genomics of SL interactions: Enables identification and transfer of SL relationships across bacterial genomes via homologous mapping.
• Study of genetic redundancy and essentiality: Facilitates analysis of essentiality patterns in duplicated genes versus singletons, exemplified by S. cerevisiae data.
• Reference dataset for experimental studies: Serves as a consolidated dataset of validated and predicted SL/SR pairs for hypothesis generation and experimental planning.

Methodology:

Putative SL pairs were identified using homologous transfer across genomes; BLAST searches were used for sequence-based mapping and homology detection; S. cerevisiae analyses compared essentiality between duplicated genes and singletons.