MultiDisp

MultiDisp analyzes and visualizes multiple sequence alignments (MSAs) to elucidate patterns of disease-causing mutations in protein kinase domains using KinMutBase mutation data.

Key Features:

• Comprehensive Mutation Database: Integrates KinMutBase entries covering 582 distinct mutations across 1,790 cases in 1,322 families with annotations at DNA, mRNA, and protein levels.
• Extensive Cross-Referencing: Links mutation entries to relevant literature and external databases for research validation and cross-checking.
• Statistical Mutation Studies: Performs statistical analyses of mutation frequencies at both DNA and protein levels with emphasis on serine/threonine kinases (PSKs) and tyrosine kinases (PTKs).
• Structural Insights: Maps mutations onto three-dimensional protein structures to reveal clustering in conserved subdomains and at substrate and coligand binding sites.
• Mutation Hotspots Identification: Identifies mutational hotspots and highlights the prevalence of CpG-containing codons for arginine.
• Comparative Mutation Analysis: Distinguishes mutation patterns and types between PSKs and PTKs to illuminate their distinct biological implications.

Scientific Applications:

• Disease Research: Supports interpretation of the genetic basis of diseases by analyzing disease-associated mutations in protein kinases.
• Genetic Studies: Provides detailed mutation and structural data for evolutionary biology and genomics analyses.
• Drug Development: Informs targeting of conserved kinase subdomains and binding sites for therapeutic design against specific kinase mutations.

Methodology:

Performs sequence alignment analysis, correlates mutation data with three-dimensional protein structures, and applies statistical genetics to relate mutations to protein function and disease.