MultiPSQ

MultiPSQ integrates and analyzes multiplex-pyrograms produced by multiplex pyrosequencing to identify SNPs and polymorphisms across multiple genomic regions for pathogen typing.

Key Features:

• Multiplex Pyrogram Analysis: Handles composite profiles created from several individual pyrograms, where each pyrogram corresponds to a specific sequencing primer.
• Flexibility and Scalability: Supports an unlimited number of sequencing primers and polymorphisms to enable analysis across multiple genomic regions.
• Enhanced Pathogen Typing: Enables SNP-based pathogen typing by considering multiple genomic regions simultaneously to improve identification of genetic variations across strains.

Scientific Applications:

• Pathogen Typing: Applicable in molecular epidemiology for identification and differentiation of closely related pathogens by analyzing multiple SNPs across genomic regions.
• Clinical Diagnostics: Usable on clinical samples and demonstrated in a proof-of-concept study involving orthopoxviruses to identify strain-specific SNPs.

Methodology:

Integrates outputs of multiple sequencing primers into a unified composite pyrogram representing the cumulative sequence information and processes data from any number of primers covering various polymorphisms.