MuPlex

MuPlex designs multiplex PCR assays to enable high-throughput, specific amplification and genotyping of thousands of target sequences such as single nucleotide polymorphisms (SNPs).

Key Features:

• Automated High-Throughput Design: Handles large-scale multiplex PCR assay design to cover thousands of single nucleotide polymorphisms (SNPs).
• User-Specified Parameters: Accepts DNA sequences and user-defined primer selection criteria, interaction parameters, and desired multiplexing levels to guide assay design.
• Multiple Solution Alternatives: Produces multiple alternative assay designs to enable exploration of trade-offs among competing objectives.
• Comprehensive Computational Analysis: Performs computational screening of primer-pair cross interactions to improve specificity and efficiency of designed assays.

Scientific Applications:

• Infectious Disease Detection: Rapid identification of multiple pathogens in clinical samples via multiplexed targets.
• Genomic Research: Facilitating whole-genome sequencing projects and closure of genomic data gaps through targeted amplification.
• Forensic Science: Enhancing accuracy and efficiency of genetic profiling in forensic investigations.
• Genotyping: High-throughput, cost-effective genotyping of large numbers of SNPs.

Methodology:

Uses computational algorithms that integrate user-defined DNA sequences, primer selection criteria, interaction parameters, and multiplexing levels to generate optimized multiplex PCR assay designs and to screen primer-pair cross interactions.